Hepatitis B Profile Test

1. Objective:

The objective of the Hepatitis B profile test was to detect and evaluate infection status, immune response, and chronicity of Hepatitis B virus (HBV) in a patient.

2. Principle:

The test was based on ELISA (enzyme-linked immunosorbent assay) or rapid immunochromatographic methods, which detected HBV antigens and antibodies using specific binding and visual color development.

3. Materials:

Patient’s blood sample (serum or plasma)

HBV profile test kit (ELISA or rapid strip)

Micropipette and tips

Test tubes and reagents

Incubator (for ELISA)

ELISA reader (if applicable)

Gloves and protective gear

4. Proceduer

1. Blood was collected aseptically and serum was separated.

2. Test strips or ELISA wells were prepared with required reagents.

3. Serum was added to each test zone (e.g., HBsAg, HBeAg, anti-HBs, anti-HBe, anti-HBc).

4. The test was incubated (e.g., 15–30 mins for rapid tests or 1–2 hours for ELISA).

5. Color development or band formation was observed.

6. The presence or absence of each marker was recorded.

5. Result:

Marker Meaning

HBsAg (+) Active infection (acute or chronic)

HBeAg (+) High infectivity

Anti-HBc (+) Previous or ongoing infection

Anti-HBs (+) Immunity (past infection or vaccination)

Anti-HBe (+) Recovery or low infectivity phase

6. Uses:

Diagnose acute or chronic Hepatitis B infection

Monitor disease stage and infectivity

Screen for HBV in blood donors

Determine immunity post-vaccination

Guide treatment and monitoring decisions

7. Consultation:

Patients with positive HBsAg were advised to undergo further tests such as HBV DNA PCR, liver function tests (LFTs), and liver ultrasound. Proper medical guidance, antiviral therapy (if needed), and lifestyle changes were recommended.